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Polyclonal Antibody Development

All polyclonal projects are conducted in our USDA Class R Research facility that is also NIH Assurance (OLAW) registered.  All MBS polyclonal products meet the EC Regulation 1069/2009 regulations allowing antibody to be shipped to Europe.  Dedicated scientists will manage your project from beginning to end using 15 years experience with a large variety of antigens and dedication to proper animal husbandry.

Immunizations:

  • Our customizable immunization protocol is designed to increase antibody production, as well as increase antibody affinity

  • Immunizations generally occur every 3 weeks.  The first bleed is taken 10 days after the second immunization

  • For an antigen less than 20 kDa, MBS recommends conjugation to a carrier protein such as KLH to increase immunogenicity and achieve the required immune response in the animal

Test Bleeds and Titer Determination:

  • Test Bleeds are taken 10 days after each immunization and screened by ELISA

  • ELISA results are graphed and sent to the customer for review

  • Test bleeds can be sent to the customer for confirmatory screening

Production Bleeds:

  • Collection of production bleeds begins upon consultation with the customer when the screening data indicates that the animal has produced a high enough titer for the end application; typically around month 4

  • Production bleeds, of 12-25 mL each per rabbit and 350-450 mL each per goat or sheep, are collected until the required amount of antiserum is achieved.  Production bleeds are taken at days 10 and 17 after immunizations

  • Purification by Protein A or G, as well as antigen specific affinity columns, is available

If a custom approach is needed for your antigen, please contact us.  Modifications such as adjuvant, boosting schedule and antigen modifications can be provided.  MBS will partner with you to provide the best chance of success for your antigen.         

Rabbit Polyclonal Antibody Development

  • NZW rabbits are purchased and housed at our USDA licensed facility

  • 5 mgs of antigen are required for an average length project

  • Standard 2 Rabbit Polyclonal Development $3,000 and up

Goat and Sheep Polyclonal Antibody Development

  • Goats and sheep are bred and raised at our USDA facility

  • 6-7 mgs of antigen required for an average length of project

  • Goats and sheep can safely be bled 2-3 times per month on a production bleed schedule.

  • Standard Goat or Sheep Polyclonal Antibody Development $3,000 and up

Llama Polyclonal Antibody Development

  • Llamas are housed at our USDA facility

  • Llamas can safely be bled 2 times per month on a production bleed schedule.

  • Standard Llama Polyclonal Antibody Development $3,600 and up

In addition to producing conventional IgG1 antibody, llamas also produce IgG2 and IgG3 isotypes which do not associate with light chains. These heavy-chain antibodies typically constitute approximately 50% of the IgG in llama serum and use a single chain variable domain to bind an epitope.

Advantages of heavy-chain llama antibodies:

  • Ability to recognize epitopes that may be inaccessible to conventional antibodies due to their smaller size and more flexible structure

  • Rapid tissue penetration with the ability to cross the blood-brain barrier.

  • High tolerance to extreme temperature and pH exposure

Fill out the Polyclonal Antibody Production Checklist and email or fax it to MBS.

Polyclonal Serum Purification

MBS offers GMP purification from our Portland, Maine facility. Protein A affinity chromatography is used for purification of rabbit serum; while Protein G is used for goat and sheep serum. Affinity chromatography with immobilized Protein A or Protein G isolates the total IgG fraction; removing serum proteins, as well as IgM antibodies.  MBS also offers affinity purification using a custom column prepared with your immobilized antigen to isolate antigen-specific antibodies. Polishing columns can also be prepared to remove a specific class of undesirable sample components and enrich the specificity of the final preparation.  Our scientists can help you strategize an optimum purification process to suit your application.