February 14th, 2013
Monoclonal Antibody to Maltose Binding Protein (MBP)
Maltose Binding Protein (MBP) tags have demonstrated utility to increase expression and proper folding of proteins expressed in bacteria leading to improved solubility of the partner protein.
Maine Biotechnology Services Inc has developed a monoclonal antibody specific for Maltose Binding Protein (MBP) to optimize detection and purification of recombinant proteins utilizing MBP tags. MAB231P has been confirmed by Western Blot and ELISA to recognize MBP irrespective of the vector used for expression.
Several recent publications bring to light the superior performance of maltose binding protein in a variety of applications including, the ability of MBP to improve soluble expression(1), act as a carrier and delivery system for fused proteins to target antigens(2), increasing the solubility of drug candidates(3), and even experimental therapeutic uses(4).
1.The ability to enhance the solubility of its fusion partners is an intrinsic property
of maltose-binding protein but their folding is either spontaneous or chaperone-mediated.
Raran-Kurussi S, Waugh DS.
2. Maltose-binding protein is a potential carrier for
oral immunizations, Veterinary Immunology and Immunopathology,
P.Bellot,P.Tiels, V.Melkebeek, B.Devriendt, B.M.Goddeeris, E. Cox,
4. Synergistic antitumor effects of Escherichia coli maltose
binding protein and Bacillus Calmette–Guerin in a mouse lung carcinoma model
Qingyong Zhang, Weihua Ni, Xiaoxia Zhao, Fengli Wang, Zhuo Gao, Guixiang Tai,
MBS developed a mouse monoclonal antibody against a polyhistidine (His) tag. MAB230P shows
high affinity, excellent functionality by Western Blot, and the ability to bind to both N and C terminal
His-tags. MAB230P, has demonstrated apparent affinity of 8.85 x 10-12 with ability to detect His tagged antigen at
picolmolar concentrations. (Determined by Forte Bio Octet Red.)
1. MW Standard
2. N-term 6X-HIS TAG protein 100ng
3. N-term 6X-HIS TAG protein 25 ng
4. N-term 6X-HIS TAG protein 5 ng
5. MW Standard
6. C-term 6X-HIS TAG protein 100 ng
7. C-term 6X-HIS TAG protein 25 ng
8. C-term 6X-HIS TAG protein 5 ng