In Vivo Monoclonal Antibody Production via Mouse Ascites (6-8 Weeks)

• All incoming cell lines require mycoplasma testing
• IFA primed mice are injected with a hybridoma cell line
• BALB/c, BALB/c hybrid, Nude and SCID mice are available; additional strains available upon request
• Ascites fluid develops over the next 10-14 days and  is harvested over multiple taps
• Antibody concentration typically increases with each successive tap
• Ascites is pooled, centrifuged, and gauze filtered
• An average yield from BALB/c mice is 3-5mLs of ascites per mouse with an average antibody concentration of 3-5mg/mL

Production Services Include:

• Expansion and cryopreservation of a hybridoma cell line to 5 vials
• Aggregate filtration of the pooled ascites
• Testing of ascites for protein content, Ig concentration
• Storage of ascites at -20C
• GMP production report including copies of zonal electrophoresis and densitometry scans

Fill out an Ascites Production Service Request form

Antibody Purification Services (1-2 weeks)

• Murine Monoclonal IgGs are purified by Protein A affinity chromatography, eluting by pH gradient to ensure gentlest conditions possible (read more)
• Concentration/dialysis to desired final buffer
• Testing of purified antibody for protein content, Ig concentration
• Sterile filtration of final product
• Addition of NaN3 upon request

Additional Services Include:

• Percent purity is determined by Size Exclusion Chromatography allowing differentiation between monomeric, dimerized, aggregated and fragmented antibody
• IsoElectric point determination  to reveal isoforms or variants in the purified material

In Vitro Production

Roller Bottle Productions or Spinner Flasks

• Cultures are grown to a specific viability and then harvested for optimal cell or cell product preparation.
• Production sizes range from 1.0L to 100.0L
• GMP production report and copy of zonal electrophoresis
• Expansion and cryopreservation of cell lines to 5 vials

Antibody Purification Services (1-2 weeks)

• Murine Monoclonal IgGs are purified by Protein A affinity chromatography, eluting by pH gradient to ensure gentlest conditions possible (read more)
• Concentration/dialysis to desired final buffer
• Testing of purified antibody for protein content, Ig concentration
• Sterile filtration of final product
• Addition of NaN3 upon request

Additional Services Include:

• Percent purity is determined by Size Exclusion Chromatography allowing differentiation between monomeric, dimerized, aggregated and fragmented antibody,
• IsoElectric point determination  to reveal isoforms or variants in the purified material.