cGMP Ascites Production and Antibody Purification Services
Ascites production is the most cost effective method of generating antibody
from your hybridoma cell line. At MBS, primed adult Balb/c, or any other
requested strain of mouse, are injected with your cell line. As a result,
ascites fluid develops over the next 10-14 days and is harvested. With each
successive tap, the quantity of ascites fluid decreases while the antibody
concentration increases. The last ascites collection contains most of your
antibody. The pooled ascites taps are then clarified by centrifugation and
gauze filtration. Average Ascites volumes are 3-5mL/mouse at an expected
antibody concentration of 3-5mg/mL.
Fill out the Ascites Production Service Request Form and email or fax back
to MBS.
All MBS cGMP Ascites production services include:
· Expansion and Cryopreservation of hybridoma cell lines to 5 vials
and first year storage
· Sequential tapping of mice
· Aggregate filtration of pooled collected ascites
· Testing of ascites for protein content, Ig concentration
· Storage of ascites at -20 degrees C
· GMP production report includes copies of zonal electrophoresis and
densitrometry scans
Mice offered include: Balb/c, C57/b1, CAF1, Balb/c hybrid, Nude and SCID
Ascites Purification Services
Murine monoclonal IgGs are purifed from ascites or in vitro technology by protein A or G affinity chromatography. MBS purifications are eluted by pH gradient to ensure the gentlest conditions possible. (read more about our purification methods in News section)
· Purification to yield final antibody amounts Concentration/dialysis
to desired transport buffer
· Testing of purified antibody for protein content, Ig concentration
· Sterile filtration of final product
· Addition of NaN3 upon request
· Percent Purity determined by Size Exclusion Chromatography allowing
differentiation between monomeric, dimerizedaggregated, and fragmented
antibody.
· IsoElectric point determination
Click here to initiate your purification with the Purification Service Request Form.