Polyclonal Antibody Development in Rabbits, Goats or Sheep

• Immunizations:
  • Our customizable immunization protocol is designed to increase antibody production, as well as increase antibody affinity
  • Immunizations occur every 3 weeks.  The first bleed is taken 10 days after the second immunization
  • If the antigen is less than 20kD, MBS recommends conjugation to a carrier protein such as KLH to produce the required immune response in the animal
• Test Bleeds and Titer Determination:
  • Test Bleeds are taken 10 days after each immunization and screened by ELISA
  • ELISA results are graphed and sent to the customer for review
  • Test bleeds can be sent to the customer for confirmatory screening
• Production Bleeds:
  • Production bleeds, of 12-25mL each per rabbit and  350-450mL each per goat or sheep, are collected until the required amount of antiserum is achieved.  Production bleeds are taken at days 10 and 14 after immunizations
  • Collection of production bleeds begins when the screening data indicates that the animal has produced a high enough titer for the end application; typically around month 4

• Purification by Protein A or G, as well as antigen specific affinity columns, is available
• USDA Class R Research Facility
• NIH Assurance (OLAW) registered facility
• EC Regulation 1774/2002 Approved Blood Collection facility enabling the antibody to be shipped into Europe

Which animal should I choose for my polyclonal antibody project?

Rabbit Polyclonal Antibody Development

• SPF Rabbits are purchased and housed at our USDA licensed facility.
• Standard 2 Rabbit Polyclonal Development $3,000 and up

Goat and Sheep Polyclonal Antibody Development

• Goats and sheep are bred and raised at our USDA facility
• Goats and sheep can safely be bled 2 times per month once they are on a production bleed schedule.
• Standard Goat or Sheep Polyclonal Antibody Development $3,000 and up

Fill out the Polyclonal Antibody Production Checklist and email or fax it to MBS.